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Synthesis and characterization of novel quercetin-α-d-glucopyranosides using glucansucrase from Leuconostoc mesenteroides
- Source :
- Enzyme and Microbial Technology. 40:1124-1129
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- In this study, two quercetin-α- d -glucopyranosides were synthesized via the acceptor reaction of a glucansucrase obtained from Leuconostoc mesenteroides B-1299CB, with quercetin and sucrose. The two transfer products were purified via HPLC, and the structures of the products were identified as quercetin-4′-O-α- d -glucopyranoside (Q-G1) and quercetin-3′-O-α- d -glucopyranoside (Q-G1′), in accordance with the results of 1H, 13C, HSQC, H-H COSY, HMBC analyses. The primary product (Q-G1) evidenced slower effects on DPPH radical-scavenging activity (SC50 = 25.2 μM) than was seen with quercetin (SC50 = 6.5 μM). The water solubility of Q-G1 was 12.7 mM, whereas the quercetin was barely soluble in water. The Ki value of Q-G1 (674.5 μM) was almost identical to that of quercetin (673.3 μM) with regard to tyrosinase inhibition effects.
- Subjects :
- chemistry.chemical_classification
biology
DPPH
Stereochemistry
Tyrosinase
Bioengineering
biology.organism_classification
Applied Microbiology and Biotechnology
Biochemistry
High-performance liquid chromatography
chemistry.chemical_compound
Enzyme
chemistry
Leuconostoc mesenteroides
Glucansucrase
biology.protein
Organic chemistry
Solubility
Quercetin
Biotechnology
Subjects
Details
- ISSN :
- 01410229
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Enzyme and Microbial Technology
- Accession number :
- edsair.doi...........ca999a6a608ebacf0a0ed45cb171936c