Alignment of Tilt Series

Computing of three-dimensional reconstructions from images obtained by transmission electron tomography needs three main steps: data acquisition, projection alignment, and 3D reconstruction. In this chapter we will focus on the process of alignment moving from the justification of its need to the study of the different classical approaches (cross-correlation, use of added fiducial markers) that have been commonly used in this alignment process. We will also discuss the most recent algorithms (multiscale registration, invariant feature recognition) as they have been adapted to Electron Tomography and improved to increase the accuracy and resolution of the final tomograms.