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Isolation and identification of a novel LCI like antibacterial protein from Bacillus sp. MD-5 reveals its potential application in controlling Staphylococcus aureus in food industry
- Source :
- Food Control. 89:142-149
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- A novel LCI like antibacterial protein produced by Bacillus sp. MD-5, which was isolated from the Arctic Ocean sediment sample during the sixth Chinese National Arctic Expedition in 2014, was purified and characterized. After saturation of Bacillus sp. MD-5 culture supernatant with ammonium sulfate and separation with ion-exchange chromatography and high performance liquid chromatography (HPLC), an antibacterial protein (MD) was purified. The MD protein was determined to be a novel LCI like antibacterial protein with molecular weight of about 7.0 kDa using liquid chromatograph-mass spectrometer (LC-MS) identification and the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). It displayed antibacterial activity against Staphylococcus aureus at minimum inhibitory concentration (MIC) of 7.33 μg/mL. MD protein possessed high thermostability and wide range of pH tolerance, and had a certain extent of protease, surfactant and metal ion resistance. MD protein was suggested to inhibit the growth of S. aureus through inhibiting peptidoglycan biosynthesis in the view of TEM detection. These results suggested that MD may represent a novel antibacterial protein with potential application in controlling S. aureus in food industry.
- Subjects :
- 0301 basic medicine
Gel electrophoresis
Protease
Chromatography
Sodium
medicine.medical_treatment
chemistry.chemical_element
medicine.disease_cause
High-performance liquid chromatography
03 medical and health sciences
Minimum inhibitory concentration
030104 developmental biology
chemistry
Staphylococcus aureus
medicine
Antibacterial activity
Food Science
Biotechnology
Thermostability
Subjects
Details
- ISSN :
- 09567135
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Food Control
- Accession number :
- edsair.doi...........c8c1deb71f4c27530db029e9be746d67