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A novel cold-inducible promoter, PThCAP from Tamarix hispida, confers cold tolerance in transgenic Arabidopsis thaliana
- Source :
- Journal of Forestry Research. 29:331-337
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Our previous studies have revealed that the ThCAP gene plays a vital role in transgenic Populus (P. davidiana × P. bolleana) in response to cold stress. However, the regulatory mechanism of ThCAP gene expression has been unclear. In this study, the 5′ flanking region of the ThCAP promoter (PThCAP) was cloned using a genome-walking method. By analyzing cis-acting regulatory elements of PThCAP, a DRE motif and MYC and MYB elements were found to be located in the promoter. To identify the regulatory elements that control the expression of the ThCAP gene promoter, a series of deletion derivatives of PThCAP, P1–P5, from the translation start code (−1538, −1190, −900, −718 and −375 bp), were fused to the GUS reporter gene, and then each deletion was stably introduced into Arabidopsis thaliana plants. Deletion analysis of the promoter suggested that only the P2 fragment had strong GUS expression in leaves and roots of A. thaliana exposed to low temperature stress. These results suggest that this 290-bp region (−1190 to −900 bp), as an important part in PThCAP, was associated with cold tolerance of A. thaliana. Our results provide evidence for the regulatory mechanism of ThCAP gene involved in the response to cold stress, and that the gene is promising candidate gene for genetic improvement of crops.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Genetics
Candidate gene
biology
Transgene
fungi
Forestry
GUS reporter system
Promoter
biology.organism_classification
01 natural sciences
03 medical and health sciences
030104 developmental biology
Tamarix hispida
Gene expression
MYB
Gene
010606 plant biology & botany
Subjects
Details
- ISSN :
- 19930607 and 1007662X
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Journal of Forestry Research
- Accession number :
- edsair.doi...........c748eccfb1e48d8d9ee42103424d3996