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Identification of wheat-Thinopyrum intermedium alien disomic addition lines conferring resistance to stripe rust

Authors :
Chun-Yu Zhang
Xianming Chen
Lijun Zhang
Bo Qu
Yu-Feng Xu
Quan Sun
Li Nan
Feng Lin
Zhai Qiang
Shi-Chang Xu
Miao Qing
Source :
Canadian Journal of Plant Science. 89:569-574
Publication Year :
2009
Publisher :
Canadian Science Publishing, 2009.

Abstract

Thinopyrum intermedium carries many useful traits for wheat genetic improvement. To identify genes conferring resistance to stripe rust caused by Puccinia striiformis f. sp. tritici in Zhong4, one of the Zhong series of partial amphiploids from the hybrids of common wheat × Th. intermedium, a cross was made between Zhong4 and a Chinese Spring ph1b mutant (CS-ph1b). The parents, F1, F2 plants and F5 lines of the cross were tested with Chinese predominant race CYR31 of P. striiformis f. sp. tritici in the seedling stage under controlled greenhouse conditions. Of 201 F2 seedlings tested with race CYR31, 149 were resistant and 52 were susceptible. The segregation fit a 3 resistant:1 susceptible ratio, indicating that a single dominant gene conferred resistance to the race CYR31. This result was further confirmed by the segregation of F5 lines into 1:1 ratio (P = 0.77). Using the Schiff dyeing method, the chromosome number in the root-tip cells of 10 F5 resistant lines (total 42 plants) was determined. Two alien addition lines (5-1-2-1-1-5, 5-1-2-1-1-6) with the smallest chromosome number (2n = 42 + II) were identified. A total of 105 pollen mother cells were examined at metaphase 1 (PMCs MI) for meiotic chromosome pairing. The lines 5-1-2-1-1-5 and 5-1-2-1-1-6 showed regular meiosis, exhibiting 22 ring or rod bivalents (2n = 22 II). The GISH results indicated that lines 5-1-2-1-1-5 and 5-1-2-1-1-6 were wheat-Th. intermedium alien disomic addition lines with 21 pairs of wheat chromosomes and one pair of Th. intermedium chromosomes. These two lines were all resistant to stripe rust, suggesting that they can be used as donors of stripe rust resistance genes and have great potential to play important roles in wheat stripe rust resistance breeding programs in China and the world.Key words: Wheat-Thinopyrum intermedium, stripe rust, GISH, cytological identification

Details

ISSN :
19181833 and 00084220
Volume :
89
Database :
OpenAIRE
Journal :
Canadian Journal of Plant Science
Accession number :
edsair.doi...........c5d3b90fe1256387c73bcdad84bedf16
Full Text :
https://doi.org/10.4141/cjps08139