Back to Search
Start Over
Development of selection marker-free transgenic potato plants with enhanced tolerance to oxidative stress
- Source :
- Journal of Plant Biology. 51:401-407
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- A binary vector devoid of a plant selection-marker gene (designated as pSSA-F) was constructed to overcome bio-safety concerns about genetically modified plants. This vector carried chloroplast-targeted superoxide dismutase (SOD) and ascorbate peroxidase (APX) genes under the control of an oxidative stress-inducible(SWPA2) promoter, and was utilized to transform potato (Solanum tuberosum L.). Integration of these foreign genes into transgenic plants was primarily performed via PCR with genomic DNA. Twelve marker-free transgenic lines were obtained by inoculating stem explants. The maximum transformation efficiency was 6.25% and averaged 2.2%. Successful integration of the SOD and APX genes rendered transgenic plants tolerant to methyl viologen-mediated oxidative stress at the leaf-disc and whole-plant levels. Our findings suggest that this technique for developing selection marker-free transgenic plants is feasible and can be employed with other crop species.
Details
- ISSN :
- 18670725 and 12269239
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Journal of Plant Biology
- Accession number :
- edsair.doi...........c536711a92c35fa3f492e65b6d460dc2