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Virus-induced gene silencing (VIGS) in Lilium leichtlinii using the Cucumber mosaic virus vector
- Source :
- Plant Biotechnology. 33:373-381
- Publication Year :
- 2016
- Publisher :
- Japanese Society for Plant Cell and Molecular Biology, 2016.
-
Abstract
- Lilies (Lilium) are among the most important floriculture crops, and to accelerate research regarding lily genetics, the development of reverse-genetics tools is necessary. However, Agrobacterium-mediated transformation in Lilium is time-consuming, since the plants require several years to progress from acclimation to flowering. Thus, virus-induced gene silencing (VIGS) is an attractive method for assaying gene function. In the present study, we modified a lily-derived strain of Cucumber mosaic virus (CMV-HL) as a VIGS vector and evaluated its effectiveness for inducing gene silencing in Lilium leichtlinii by introducing L. leichtlinii phytoene desaturase (LlPDS) gene fragments into an intercistronic region between the 3a and 3b genes of the CMV-HL RNA3 genome. At 30 days after inoculation (dpi) with LlPDS-containing CMV-HL, photo-bleaching was observed in the upper leaves of L. leichtlinii, and at 57 dpi, we observed that the natural orange color in flower tepals had faded. Reduced LlPDS expression and the detection of small interfering LlPDS RNA indicated that the color changes were the result of LlPDS gene silencing. In addition, the leaves also exhibited a mild photo-bleaching phenotype in the following year. Therefore, our results indicate that CMV-HL spreads systemically in the leaves and flowers of Lilium during the first year of infection, as well as in new shoots during the following year, and that the vector system can be successfully applied to induce short-term endogenous gene silencing in lilies.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Phytoene desaturase
Lilium
biology
Plant Science
biology.organism_classification
01 natural sciences
Cucumber mosaic virus
03 medical and health sciences
Tepal
Transformation (genetics)
030104 developmental biology
Shoot
Botany
Gene silencing
Agronomy and Crop Science
Gene
010606 plant biology & botany
Biotechnology
Subjects
Details
- ISSN :
- 13476114 and 13424580
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Plant Biotechnology
- Accession number :
- edsair.doi...........c49adc4b82f9a0d29672d0c6db555b00