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Amyloid β1–42-Induced Rapid Zn2+ Influx into Dentate Granule Cells Attenuates Maintained LTP Followed by Retrograde Amnesia
- Source :
- Molecular Neurobiology. 56:5041-5050
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- On the basis of the evidence that amyloid β1-42 (Aβ1-42)-induced Zn2+ influx affects memory acquisition via attenuated long-term potentiation (LTP) induction, here we tested whether Aβ1-42-induced Zn2+ influx affects maintained LTP in freely moving rats, resulting in retrograde amnesia. Both maintained LTP and space memory were impaired by local injection of 250 μM ZnCl2 (2 μl) into the dentate gyrus, while maintained LTP was impaired by injection of either Aβ1-40 or Aβ1-42 (25 μM, 2 μl) into the dentate gyrus. Aβ1-40-induced impairment of maintained LTP was rescued by co-injection of CaEDTA, an extracellular Zn2+ chelator, but not by co-injection of ZnAF-2DA, an intracellular Zn2+ chelator, suggesting that maintained LTP is impaired by Aβ1-40 via a mechanism that may involve extracellular Zn2+. In contrast, Aβ1-42-induced impairments of maintained LTP and space memory were rescued by co-injection of either CaEDTA or ZnAF-2DA. Intracellular Zn2+ in dentate granule cells was rapidly increased by Aβ1-42 injection into the dentate gyrus, but not by Aβ1-40 injection. The block of Aβ1-42-induced increase in intracellular Zn2+ by pretreatment with dexamethasone, a metallothionein inducer also rescued Aβ1-42-induced impairment of maintained LTP. The present study indicates that Aβ1-42-induced Zn2+ influx into dentate granule cells, which more readily occurs than free Zn2+-induced Zn2+ influx, attenuates maintained LTP followed by retrograde amnesia. It is likely that controlling Aβ1-42-induced intracellular Zn2+ dysregulation is a strategy for defending AD pathogenesis.
- Subjects :
- inorganic chemicals
0301 basic medicine
biology
Chemistry
Amyloid beta
Dentate gyrus
Neuroscience (miscellaneous)
Amnesia
Retrograde amnesia
Long-term potentiation
medicine.disease
Cell biology
03 medical and health sciences
Cellular and Molecular Neuroscience
030104 developmental biology
0302 clinical medicine
nervous system
Neurology
medicine
Extracellular
biology.protein
Cognitive decline
medicine.symptom
030217 neurology & neurosurgery
Intracellular
Subjects
Details
- ISSN :
- 15591182 and 08937648
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Molecular Neurobiology
- Accession number :
- edsair.doi...........c437930f8971feb815cdb7b8b39e73ac