Evaluations of Marine Collagen Peptides from tilapia skin on experimental oral ulcer model of mice

The present study aimed to evaluate the oral ulcer healing process treated with Marine Collagen Peptides (MCPs) from tilapia skin. Oral ulcer models were established on the tongue mucosa of C57/BL6 mice by phenol liquids. 60 mice were randomly divided into four groups: blank group, normal saline group (NS), low concentration MCPs group (LMCPs) and high concentration MCPs group (HMCPs). Each substance was respectively administered on the ulcer area once daily and continued until sacrificed (after 1, 3, 5, 7 and 9 days of ulcer establishment). In addition to clinical and macroscopic evaluation, we also performed histological, histomorphometric and immunohistochemistry analysis. The results indicated that MCPs could accelerate the oral ulcer healing process effectively as a result of the high content Pro-Hyp effects. Firstly it reduced the numbers of inflammatory cells infiltration. Secondly it promoted collagenesis, angiogenesis and re-epithelialization from D5. Thirdly, it down-regulate the expressions of IL-1β and TNF-α in the mid-late stage. In general, MCPs has great potential for developing clinical applications on oral ulcer treatments.