Renocardiac Syndrome Induced by Renal Ischemia and Reperfusion: Vibrational Markers

Renal injury caused by renal ischemia and reperfusion is capable to change heart morphology, electrophysiology, and redox unbalance. The so-called cardio-renal syndrome is an important class of dysfunction since heart and kidneys are responsible for hemodynamic stability and organ perfusion through a complex network. In the present work we investigate the Fourier-Transform Raman vibrational spectral features of cardiac hypertrophy induced by renal ischemic reperfusion. C57BL/6J mice were subjected to unilateral occlusion of the renal pedicle for 60 minutes and reperfused for 5 days, 8 days, and 15 days. It was observed that bands around 540, 1100, 1300, 1450, 1650, and 2500 cm-1 dominates the spectra. They are associated to stretching of S-S in Cysteine amino acid, stretching of C-C in lipids, twisting of CH2 in collagen and phospholipids, bending modes of CH3 in lipids and amino acids side chains, Amide I vibration of proteins. The intensities of these vibrations are modulated during renocardiac syndrome. We find that tyrosine, tryptophan, cystine/cysteine, fibroblast growth factors, collagen III alterations from homeostasis were the metabolites associate with these changes. These findings are clinically relevant once the presented bands can be used as molecular markers of preventing cardiac diseases’ development in patients with renal injury.