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Global Mass Spectrometry-Based Analysis of Protein Ubiquitination Using K-ε-GG Remnant Antibody Enrichment

Authors :
Jian Min Ren
Alissa J. Nelson
Matthew P. Stokes
Yiying Zhu
Source :
Targeted Protein Degradation ISBN: 9781071616642
Publication Year :
2021
Publisher :
Springer US, 2021.

Abstract

Ubiquitination is a post-translational modification that affects protein degradation as well as a variety of cellular processes. Methods that globally profile ubiquitination are powerful tools to better understand these processes. Here we describe an updated method for identification and quantification of thousands of sites of ubiquitination from cells, tissues, or other biological materials. The method involves cell lysis and digestion to peptides, immunoaffinity enrichment with an antibody recognizing di-glycine remnants left behind at ubiquitinated lysines, and liquid chromatography-tandem mass spectrometry analysis of the enriched peptides.

Details

ISBN :
978-1-07-161664-2
ISBNs :
9781071616642
Database :
OpenAIRE
Journal :
Targeted Protein Degradation ISBN: 9781071616642
Accession number :
edsair.doi...........c2f9e71255b5905d2ba85aa282c506a7
Full Text :
https://doi.org/10.1007/978-1-0716-1665-9_11