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Temporal expression of inflammatory cytokines and chemokines in rat adjuvant‐induced arthritis
- Source :
- Arthritis & Rheumatism. 43:1266-1277
- Publication Year :
- 2000
- Publisher :
- Wiley, 2000.
-
Abstract
- OBJECTIVE To examine cytokine and chemokine production during the evolution of rat adjuvant-induced arthritis (AIA), a model of rheumatoid arthritis. METHODS Clinical and laboratory assessment of the course of AIA was performed over a 47-day period. Levels of the cytokines tumor necrosis factor a (TNFalpha), interleukin-1beta (IL-1beta), and IL-6, as well as levels of the chemokines macrophage inflammatory protein 1alpha (MIP-1alpha) and JE, the murine homolog of monocyte chemoattractant protein 1, were determined by enzyme-linked immunosorbent assay in the sera and joints of AIA and control rats. Synovia from AIA rats were (immuno)histochemically analyzed. Results of cytokine and chemokine measurements were correlated with clinical and laboratory markers of inflammation and histology. RESULTS Early (before day 14 post adjuvant injection) and later phases of AIA could be distinguished. Cytokine and chemokine production was increased in AIA versus control rats. The production of TNFalpha, IL-1beta, MIP-1alpha, and, as determined earlier, epithelial neutrophil-activating peptide 78-like protein was abundant prior to and during the course of AIA, while that of IL-6 and JE was elevated in the late phase of AIA. Cytokine and chemokine levels were correlated with the clinical symptoms of arthritis and blood neutrophil counts. Joint levels of IL-1beta showed correlation with synovial lining proliferation and neutrophil ingress into AIA synovium. CONCLUSION Cytokines and chemokines are involved in the clinical, laboratory, and histologic changes underlying AIA. The production of these mediators may be temporally and spatially regulated. These findings may be important for the optimal timing of cytokine and chemokine targeting.
- Subjects :
- musculoskeletal diseases
Pathology
medicine.medical_specialty
Chemokine
biology
business.industry
medicine.medical_treatment
Immunology
Arthritis
Inflammation
medicine.disease
Proinflammatory cytokine
Cytokine
Rheumatology
Rheumatoid arthritis
medicine
biology.protein
Immunology and Allergy
Pharmacology (medical)
Tumor necrosis factor alpha
medicine.symptom
business
Macrophage inflammatory protein
Subjects
Details
- ISSN :
- 15290131 and 00043591
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Arthritis & Rheumatism
- Accession number :
- edsair.doi...........c29d2e91ff0b2959c68c47c0a7089cf7