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Effect of electric field on liquid chromatographic separation of peptide digests
- Source :
- Journal of Chromatography A. 832:149-163
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- A system is described which allows operation of a range of capillary based liquid phase separations including capillary electrophoresis, isocratic and gradient capillary electrochromatography, isocratic and gradient capillary liquid chromatography and electrically assisted gradient capillary liquid chromatography. The system was coupled to electrospray ionization mass spectrometry in the electrically assisted capillary liquid chromatography mode to investigate the effect of applied voltage on the selectivity in peptide mapping separations. Analyses were performed on tryptic digests of recombinant human growth hormone and tissue plasminogen activator. The results show a small but useful effect on selectivity that can be used to fine tune specific separations.
- Subjects :
- Capillary electrochromatography
Electrospray
Chromatography
Chemistry
Capillary action
Elution
Organic Chemistry
Analytical chemistry
General Medicine
Biochemistry
Capillary electrophoresis–mass spectrometry
High-performance liquid chromatography
Analytical Chemistry
Capillary electrophoresis
Electrochromatography
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 832
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi...........c1b9e44ea3f11dfd6a6a9b4d1b3aefec