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A model for the interaction of the G3-subdomain ofGeobacillus stearothermophilusIF2 with the 30S ribosomal subunit
- Source :
- Protein Science. 25:1722-1733
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Bacterial translation initiation factor IF2 complexed with GTP binds to the 30S ribosomal subunit, promotes ribosomal binding of fMet-tRNA, and favors the joining of the small and large ribosomal subunits yielding a 70S initiation complex ready to enter the translation elongation phase. Within the IF2 molecule subdomain G3, which is believed to play an important role in the IF2-30S interaction, is positioned between the GTP-binding G2 and the fMet-tRNA binding C-terminal subdomains. In this study the solution structure of subdomain G3 of Geobacillus stearothermophilus IF2 has been elucidated. G3 forms a core structure consisting of two β-sheets with each four anti-parallel strands, followed by a C-terminal α-helix. In line with its role as linker between G3 and subdomain C1, this helix has no well-defined orientation but is endowed with a dynamic nature. The structure of the G3 core is that of a typical OB-fold module, similar to that of the corresponding subdomain of Thermus thermophilus IF2, and to that of other known RNA-binding modules such as IF2-C2, IF1 and subdomains II of elongation factors EF-Tu and EF-G. Structural comparisons have resulted in a model that describes the interaction between IF2-G3 and the 30S ribosomal subunit.
- Subjects :
- 0301 basic medicine
biology
Eukaryotic Large Ribosomal Subunit
Thermus thermophilus
Ribosomal RNA
biology.organism_classification
Biochemistry
03 medical and health sciences
Crystallography
030104 developmental biology
Eukaryotic translation
Eukaryotic initiation factor
Biophysics
Initiation factor
Eukaryotic Small Ribosomal Subunit
30S
Molecular Biology
Subjects
Details
- ISSN :
- 09618368
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Protein Science
- Accession number :
- edsair.doi...........c1a62c9c00946949a4e79e48937ddc2b