Application of SPR biosensor for medical diagnostics of human hepatitis B virus (hHBV)

The feasibility of a surface plasmon resonance (SPR) biosensor was tested for the medical diagnosis of human hepatitis B virus (hHBV). For the practical application of the SPR biosensor, the baseline stability and the dilution factor of serum samples was optimized. A standard curve was prepared using samples of HBV antibodies. In order to improve the sensitivity of the SPR biosensor, several amplification methods were tested using secondary antibodies, avidin-biotynylated antibodies and peroxidase–anti-peroxidase (PAP) complex. By applying the amplification methods, the detection limit of the SPR biosensor for the medical diagnosis was found to be very similar to the commercial ELISA kit for the diagnosis of hepatitis as a reference method.