146-POS

Objectives To determine, in Type 1 diabetic (T1DM) women, if kidney injury associated with subsequent pre-eclampsia (PE) is detectable early in pregnancy, before overt proteinuria develops. Methods The prospective cohort comprised 22 T1DM women who developed PE at ∼ 37 weeks, 23 T1DM women who remained normotensive, and 17 non-diabetic women. All participants had normal urinary albumin levels at study entry. Urine samples were collected at three study visits (12.2 ± 1.9, 21.6 ± 1.5, and 31.5 ± 1.7 weeks of gestation; mean ± SD), preceding PE onset. Urinary levels of neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) were measured by ELISA and normalized to urinary creatinine. Data were analyzed by ANOVA followed by post-hoc t tests, and by Generalized Estimating Equations. Results In T1DM PE vs. T1DM normotensive women, NGAL was significantly higher at V1 (P = 0.016), and non-significantly higher at V2 and V3, although the difference was not statistically significant when all three visits were considered together (p = 0.13). There was no difference between women with vs. without T1DM. KIM-1 showed a trend to higher values at V1 (P = 0.098) and across all three visits (P = 0.085) in T1DM PE vs. T1DM normotensive women; again, there was no difference between women with vs. without T1DM. Conclusions Subclinical kidney injury occurring in early pregnancy may predict PE. NGAL and KIM-1 have the potential to be sensitive early biomarkers, but need further evaluation. Disclosures M.B. Hookham: None. J.Y. Yu: None. A.J. Jenkins: None. K.F. Hanssen: None. C.E. Aston: None. T.J. Lyons: None.