Characterization of Fusarium Verticillioide Isolates from Pokkah Boeng on Sugarcane and The Disease Incidence In Field

Pokkah boengof sugarcane caused by Fusariumverticillioidesis is a destructive fungal disease in sugarcane growing regions In China the notable losses from pokkah boeng have occurred annually The main objectives of this study were to a nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp Field disease survey of natural disease incidenceof Pokkah boengin cultivars and cross combinations of sugarcane insugarcaneseedling nurserieslocated in Fuzhou China thedisease incidence ranged from b nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp Morphology characterization of F verticillioides was observed and identified by macroconidia microconidia conidiophoresand culture pigment characteristics c nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp nbsp Representing F verticillioidescausing pokkah boeng in sugarcane wasphylogenetic analyzed using translation elongation factor TEF ATP ATPase gene and internal transcribed spacer ITS gene regions Most of the isolates of the present study showed a similar grouping pattern in case of the three gene sequences however ITS and TEF more conservative than ATP ATPase gene in isolates of F verticillioides