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Cell mechanics characteristics of anti-HER2 modified PPy@GNPs and its photothermal treatment of SKOV-3 cells
- Source :
- Applied Nanoscience. 11:911-919
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Nanomaterials and nanotechnology have been widely used in various fields, such as materials, chemical engineering, optics, and other disciplines. In this paper, gold nanoparticles are prepared by the hydrothermal method and treated with polypyrrole coating (PPy-GNPs). Then, the anti-HER2 protein was conjugated to polypyrrole coated gold nanoparticles, and the nanomaterials are named anti-HER2-PPy@GNPs (anti-PPy@GNPs). Their diameter is about 80–90 nm, and they show a strong absorption peak in the near-infrared band, especially under 808 nm laser irradiation. In the MTT test experiment, anti-HER2-PPy@GNPs show a significant inhibitory effect on the SKOV-3 cell. Fluorescent cytoskeletal analysis of SKOV-3 cells and atomic force microscopy (AFM) of the mechanical characteristics of the cell surface revealed that the cell micro-structure, adhesion, and Young's modulus of anti-HER2-PPy@GNPs have changed under the near-infrared illumination. These results indicate anti-HER2-PPy@GNPs has an inhibitory effect on SKOV-3 cells. AFM detects the mechanical characteristics of SKOV-3 cells. Compared with the control group, Young's modulus is increased 52.7%, while the adhesion force is decreased 14.47%.
- Subjects :
- Materials science
Materials Science (miscellaneous)
Nanochemistry
02 engineering and technology
engineering.material
010402 general chemistry
Polypyrrole
01 natural sciences
Nanomaterials
chemistry.chemical_compound
Coating
Electrical and Electronic Engineering
Physical and Theoretical Chemistry
Cell Biology
Adhesion
Photothermal therapy
021001 nanoscience & nanotechnology
Fluorescence
Atomic and Molecular Physics, and Optics
0104 chemical sciences
chemistry
Chemical engineering
Colloidal gold
engineering
0210 nano-technology
Biotechnology
Subjects
Details
- ISSN :
- 21905517 and 21905509
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Applied Nanoscience
- Accession number :
- edsair.doi...........c001b2478987e9f494927d76aef8611c