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The Bacteriophage vB_CbrM_HP1 Protects Crucian Carp Against Citrobacter braakii Infection

Authors :
Chunzheng Huang
Chao Feng
Xiao Liu
Rihong Zhao
Zijing Wang
Hengyu Xi
Hongda Ou
Wenyu Han
Zhimin Guo
Jingmin Gu
Lei Zhang
Source :
Frontiers in Veterinary Science. 9
Publication Year :
2022
Publisher :
Frontiers Media SA, 2022.

Abstract

Citrobacter braakii is an opportunistic pathogen that induces aquatic infections in fish and turtles. In this study, a bacteriophage that infects C. braakii, named vB_CbrM_HP1, was isolated from sewage. This phage belongs to Myoviridae family, Ounavirinae subfamily, Mooglevirus genus. We also used the phage to treat crucian carp infection caused by C. braakii for the first time. vB_CbrM_HP1 was relatively stable at temperatures ranging from 4 to 60°C and pH values ranging from 3 to 11 but float slightly. When the multiplicities of infection (MOI) was 0.0001, the titer reached a maximum of 4.20 × 1010 PFU/ml. As revealed from the results of whole genomic sequence analysis, the total length of vB_CbrM_HP1 was 89335 bp, encoding 135 ORFs, 9 of which were C. braakii both in vitro and in vivo. In vitro, vB_CbrM_HP1 was capable of effectively killing bacteria (the colony count decreased by 4.7 log units at 5 h). In vivo, administration of vB_CbrM_HP1 (1 × 109 PFU) effectively protected crucian carp against fatal infection caused by C. braakii. Phage treatment reduced the levels of inflammatory factors. All these results demonstrated the potential of vB_CbrM_HP1 as an alternative treatment strategy for infections caused by C. braakii.

Subjects

Subjects :
General Veterinary

Details

ISSN :
22971769
Volume :
9
Database :
OpenAIRE
Journal :
Frontiers in Veterinary Science
Accession number :
edsair.doi...........bfad9989f7bb41d607dc1abeb57fb4f7
Full Text :
https://doi.org/10.3389/fvets.2022.888561