Additional file 4: Figure S4. of Estrogen receptor α in cancer associated fibroblasts suppresses prostate cancer invasion via reducing CCL5, IL6 and macrophage infiltration in the tumor microenvironment

Co-culture of CAF ERα(+) cells and PCa cells can decrease PCa cell invasion through changing IL-6 expression. CM was collected from CAF.ERα(-) and CAF.ERα(+) cells together with or without with IL-6 neutralizing antibody (Anti IL-6) or IgG (control) into bottom wells of 24-well transwell systems. We then trypsinized and seeded CWR22Rv-1 and C4-2 cells (1x105) into matrigel-pre-coated transwells for invasion assay. Quantitation is at right.