Brachybacterium subflavum sp. nov., a novel actinobacterium isolated from the foregut of grass carp

A novel actinobacterium, designated CFH 10395T, was isolated from the foregut of grass carp (Ctenopharyngodon idella), which had been fed with ginseng extract supplement. The taxonomic position was investigated by a polyphasic approach. Cells of CFH 10395Twere Gram-staining-positive, aerobic, ovoid-shaped, non-spore-forming and non-motile. On the basis of the results of 16S rRNA gene sequence analysis, CFH 10395Twas most closely related toBrachybacterium endophyticumKCTC 49087T,Brachybacterium squillarumJCM 16464TandBrachybacterium paraconglomeratumJCM 17781T(97.85%, 97.51 and 97.29% similarity, respectively). CFH 10395Tgrew at 4–37 °C, pH 5.0–9.0 and in the presence of up to 10.0 % NaCl (w/v). The dominant menaquinone was MK-7. The whole-cell sugars were rhamnose, glucose, mannose and galactose.meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0and iso-C16 : 0. The genome size was 3.99 Mbp with a DNA G+C content of 71.9 mol%. On the basis of the results of phylogenetic analysis, physiological properties, chemotaxonomic characteristics, low average nucleotide identity (ANI) and digital DDH (dDDH) results [ANI calculated using MUMmer (ANIm) blast(ANIb) Trepresents a novel species of the genusBrachybacterium, for which the nameBrachybacterium subflavumsp. nov., is proposed. The type strain is CFH 10395T(=CGMCC 1.13804T=KCTC 49235T).