Virucidal activity of disinfectant ‘Biolaid’

The article presents the results of the study of toxic and virucidal action of the disinfectant ‘Biolaid’, which includes hydrogen peroxide, lactic acid, and supralactic acid. The research was conducted following the ‘Methodical Approaches to the Control of Disinfectants for Veterinary Medicine’ (Kovalenko and Nedosiekov, 2011). Toxicity of the disinfectant ‘Biolaid’ was characterized in SPEV and BHK-21/C13 cell cultures (ATCC CCL-10). Determination of virucidal activity of the disinfectant ‘Biolaid’ was performed on models of Aujeszky’s disease virus (strain ‘Arsky’) and rabies virus (strain CVS-11, ATCC VR 959). The toxic effect of the drug ‘Biolaid’ was determined for concentrations of 2.0%, 1.5%, 1.0%, 0.5%, and 0.25% at exposures of 30 and 60 min in an incubator at 37°С. The virucidal effect of ‘Biolaid’ was determined for similar concentrations using working dilutions of viral suspensions: for Aujeszky’s disease virus — 4.0 CPE50/cm3, for rabies virus — 4.0 TCID50/cm3. The results of the study showed that the disinfectant ‘Biolaid’ is not toxic to SPEV and BHK-21/C13 cells in all test concentrations (2.0%, 1.5%, 1.0%, 0.5%, and 0.25%) at exposures of 30 and 60 min. Disinfectant ‘Biolaid’ has 100% virucidal activity against Aujeszky’s disease virus (strain ‘Arsky’) and rabies virus (strain CVS-11, ATCC VR 959) in all tested concentrations (2.0%, 1.5%, 1.0%, 0.5%, and 0.25%). The virucidal effect of these viruses was manifested at exposures of both 30 and 60 min. The obtained results give grounds to recommend disinfectant ‘Biolaid’ for disinfection of various livestock and poultry farms in case of detection of viral infections