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Development of a tier 1 R5 clade C simian-human immunodeficiency virus as a tool to test neutralizing antibody-based immunoprophylaxis
- Source :
- Journal of Medical Primatology. 40:120-128
- Publication Year :
- 2010
- Publisher :
- Wiley, 2010.
-
Abstract
- Background While some recently transmitted HIV clade C (HIV-C) strains exhibited tier 1 neutralization phenotypes, most were tier 2 strains (J Virol 2010; 84:1439). Because induction of neutralizing antibodies (nAbs) through vaccination against tier 2 viruses has proven difficult, we have generated a tier 1, clade C simian–human immunodeficiency virus (SHIV-C) to permit efficacy testing of candidate AIDS vaccines against tier 1 viruses. Methods SHIV-1157ipEL was created by swapping env of a late-stage virus with that of a tier 1, early form. Results After adaptation to rhesus macaques (RM), passaged SHIV-1157ipEL-p replicated vigorously in vitro and in vivo while maintaining R5 tropism. The virus was reproducibly transmissible intrarectally. Phylogenetically, SHIV-1157ipEL-p Env clustered with HIV-C sequences. All RM chronically infected with SHIV-1157ipEL-p developed high nAb titers against autologous as well as heterologous tier 1 strains. Conclusions SHIV-1157ipEL-p was reproducibly transmitted in RM, induced cross-clade nAbs, and represents a tool to evaluate anti-HIV-C nAb responses in primates.
- Subjects :
- General Veterinary
biology
viruses
education
virus diseases
Simian immunodeficiency virus
AIDS Vaccines
medicine.disease_cause
Virology
Virus
Neutralization
Titer
health services administration
biology.protein
medicine
Animal Science and Zoology
Antibody
Neutralizing antibody
health care economics and organizations
Tropism
Subjects
Details
- ISSN :
- 00472565
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Primatology
- Accession number :
- edsair.doi...........be042a873f2154057415a70ea3559648
- Full Text :
- https://doi.org/10.1111/j.1600-0684.2010.00454.x