Cytoprotective Effects of Jaboticaba (Pliniaperuviana, Poir. Govaerts) Fruit Peel Extracts against H2O2-Induced Oxidative Stress

Oxidative stress is a result of disturbance between the production of reactive oxygen species (ROS) and antioxidants defenses. Plant secondary metabolites can be used as exogenous antioxidants to prevent human oxidative stress-related diseases. This study aimed to determine the antioxidant and cytoprotective effects of jaboticaba fruit peel hydroalcoholic extracts against H 2 O 2 -induced oxidative stress in a murine fibroblast (L929 cell line) model. Ultra-high pressure hydroalcoholic extracts (50% ethanol, UP) were obtained over 4 h, with 1 h interval between collections. UP 3 h and UP 4 h extracts presented the highest and the lowest phenolic and flavonoid contents, respectively. Antioxidant capacity showed that 1 mg/ml extracts inhibited 90% of DPPH radical activity and UP 1 h extract had significant (p < 0.05) higher scavenging activity than other extracts at this concentration. After 24 h of exposure to UP 4 h extracts, cell viability was significantly increased when compared to control, UP 1 h, and UP 2 h extracts. When cells were previously treated with UP hydroalcoholic extracts followed by H 2 O 2 exposure, all extracts at 0.05 and 0.1 mg/ml exhibited significant (p < 0.05) cytoprotective effects in relation to control. Results demonstrated that jaboticaba fruit peel extracts are antioxidants and exert cytoprotective effects under H 2 O 2 -induced oxidative stress.