Gel permeation of human serum lipoprotein by HPLC

Human serum (plasma) lipoproteins were fractionated into four or five classes using the ultracentrifugation technique of O. F. de Lalla and J. W. Gofman. The particle sizes of serum lipoprotein fractions are quite different from each other resulting in the application of gel permeation chromatography using agarose gel by T. Sata et al. and hydroxyapatite by G. M. Kostner et al. The reference standards of human serum lipoprotein are prepared by the sequential ultracentrifugal procedure of R. J. Havel et al. Each reference standard is assayed for its homogeneity by immunoelectrophoresis. R. M. Carrol and L. L. Rudel compared the separation patterns of total lipoprotein fraction with different combinations of column by monitoring the absorption at 280 nm. The shapes of the patterns drawn by triglyceride, total cholesterol, and phospholipid measurements accurately reflect the different contents of each lipid constituent in each lipoprotein fraction.