Anti-inflammatory effect of microalgal extracts from Tetraselmis suecica

The aim of the present study was to examine the anti-inflammatory activities of extracts from Tetraselmis suecica with respect to nitric oxide (NO) production, tumor necrosis factor (TNF)-α and interlukin (IL)-6 release in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. We prepared methanolic extracts and water extract using protease. Of all the prepared extracts, 80% methanol extract exhibited the strongest anti-inflammatory effect. Thus, we further characterized the hexane fraction (He-TS) and ethyl acetate fraction (EA-TS) that contained potential bioactive compounds from the 80M-TS fraction of T. suecica. Both He-TS and EA-TS fractions resulted in a significant and dose-dependent inhibition of LPS-induced NO production, TNF-α, and IL-6 release (p