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Novel pH-Sensitive Microbial Rhodopsin from Sphingomonas paucimobilis
- Source :
- Doklady Biochemistry and Biophysics. 495:342-346
- Publication Year :
- 2020
- Publisher :
- Pleiades Publishing Ltd, 2020.
-
Abstract
- This work provides the first characteristics of the rhodopsin SpaR from Sphingomonas paucimobilis, aerobic bacteria associated with opportunistic infections. The sequence analysis of SpaR has shown that this protein has unusual DTS motif which has never reported in rhodopsins from Proteobacteria. We report that SpaR operates as an outward proton pump at low pH; however, proton pumping is almost absent at neutral and alkaline pH. The photocycle of this rhodopsin in detergent micelles slows down with an increase in pH because of longer Schiff base reprotonation. Our results show that the novel microbial ion transporter SpaR of interest both as an object for basic research of membrane proteins and as a promising optogenetic tool.
- Subjects :
- 0303 health sciences
Sphingomonas paucimobilis
biology
Chemistry
Aerobic bacteria
030302 biochemistry & molecular biology
Biophysics
General Chemistry
General Medicine
biology.organism_classification
Biochemistry
Micelle
03 medical and health sciences
Membrane protein
Rhodopsin
biology.protein
Spar
Proteobacteria
Ion transporter
030304 developmental biology
Subjects
Details
- ISSN :
- 16083091 and 16076729
- Volume :
- 495
- Database :
- OpenAIRE
- Journal :
- Doklady Biochemistry and Biophysics
- Accession number :
- edsair.doi...........b9d558933cff77b7fd9791461e728069