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Fractionation and Characterization of Mitogenic and Anti-Complementary Active Fractions from Kampo (Japanese Herbal) Medicine 'Juzen-Taiho-To'1
- Source :
- Planta Medica. 56:386-391
- Publication Year :
- 1990
- Publisher :
- Georg Thieme Verlag KG, 1990.
-
Abstract
- "Juzen-Taiho-To" (TJ-48), which is a kampo (Japanese herbal) medicine prepared by decocting a prescription of ten kinds of herbs, has several immunostimulating activities. In order to characterize the active substances for anti-complementary and mitogenic activities, TJ-48 was fractionated. Anti-complementary activity was observed in the water- and methanol-insoluble fraction (F-2) and the crude polysaccharide fraction (F-5), whereas mitogenic activity was only found in F-5. However, other low molecular mass fractions did not show both activities. Methylation analysis indicated that F-2 mainly contained amylopectin-like polysaccharides. Both Pronase digestion and periodate oxidation decreased the anti-complementary activity of F-2, and the beta-amylase-resistant fraction of F-2 still retained the potent anti-complementary activity. When F-5, which has the most potent of both activities, was further fractionated, only the major acidic polysaccharide fraction, F-5-2, showed potent mitogenic activity. Endo-alpha-(1----4)-polygalacturonase digestion showed that F-5-2 mainly contained pectic polysaccharides, and the endo-polygalacturonase treatment of F-5-2 reduced the mitogenic activity but not the anti-complementary activity. F-2 and F-5 each activated the complement system by a different mode of action.
- Subjects :
- Pharmacology
chemistry.chemical_classification
Traditional medicine
Molecular mass
business.industry
Kampo
Organic Chemistry
Pharmaceutical Science
Biological activity
Fractionation
Pharmacognosy
Polysaccharide
Analytical Chemistry
Complementary and alternative medicine
chemistry
Drug Discovery
Molecular Medicine
Medicine
Digestion
business
Mode of action
Subjects
Details
- ISSN :
- 14390221 and 00320943
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Planta Medica
- Accession number :
- edsair.doi...........b979228b835369d8360bbb0d273e055e