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AB0029 Imbalance between Immunoregulatory and Effector TH17 Pathway in Active Sle
- Source :
- Annals of the Rheumatic Diseases. 73:813.1-813
- Publication Year :
- 2014
- Publisher :
- BMJ, 2014.
-
Abstract
- Background Defective function of regulatory T cells (Treg) leads to the impairment of immune tolerance implicated in the pathogenesis of autoimmune inflammation in systemic lupus erythematosus (SLE). Methods To investigate the role of Treg in SLE we studied peripheral blood Treg and CD4+ effector T-helper cells (Th1, Th17), expression of their signature cytokines by in vitro activated lymphocytes (real-time PCR), and levels of TGF-β1, IL-10 and IL-17A (ELISA) in sera from patients with active–SLE (n=13), inactive-SLE (n=13) and matched healthy subjects (n=13). Results There was a significant decrease in CD4+ lymphocyte count in active SLE, with no evident difference in the percentage of CD25+FoxP3+ Treg cells as compared to other groups. Treg absolute counts decreased significantly in active-SLE (∼2-fold as compared to controls) and weakly correlated with markers of disease activity (C4, r=0.44). Only Th1 (IFN-γ producing) cells, but not Th17 (IL-17A producing) cells decreased in active disease, resulting in lower Th1/Th17 ratio. This was confirmed by a lower expression of IFN-γ mRNA (but not IL-17A) in peripheral blood lymphocytes from patients with active-SLE. All patients with SLE were characterized by lower Treg/Th17 ratio (median 1.4 vs. 2.4 in controls, p Conclusions Our data suggest, that SLE associated lymphopenia affects primarily Treg and Th1, but not Th17 fraction, resulting in imbalance between regulatory and effector CD4+ subsets. This may lead to relative deficiency in suppressive function of Treg (as evidenced by decrease in TGF-β1), and consequently to the increased proinflammatory response during active phase of the disease. Disclosure of Interest None declared DOI 10.1136/annrheumdis-2014-eular.3602
- Subjects :
- business.industry
Effector
Lymphocyte
Immunology
FOXP3
chemical and pharmacologic phenomena
hemic and immune systems
General Biochemistry, Genetics and Molecular Biology
In vitro
Immune tolerance
Proinflammatory cytokine
Pathogenesis
medicine.anatomical_structure
Rheumatology
Immunology and Allergy
Medicine
IL-2 receptor
skin and connective tissue diseases
business
Subjects
Details
- ISSN :
- 14682060 and 00034967
- Volume :
- 73
- Database :
- OpenAIRE
- Journal :
- Annals of the Rheumatic Diseases
- Accession number :
- edsair.doi...........b93c13cf6455c43c0a3080bcc815a26c