Mechanical model of the physiological microenvironment of cardiomyocytes

The study of single-cell mechanical properties helps detect and recognize abnormal cells and can provide a potential method for early diagnosis of fatal diseases. Although some cell models have been proposed to explain the mechanical response, most are based on specific experimental conditions and cannot be applied to various micro-operation conditions. Developing a general cell mechanical model for a variety of experimental conditions is of great significance. A mechanical model of a physiological microenvironment based on cardiomyocytes was constructed in vitro. The biomechanical properties of AC-16 cells in different axial positions were calculated using an inverted phase-contrast microscope. The mechanical parameters of rat cardiomyocytes were measured and analyzed using green fluorescent protein and a three-dimensional magnetic twisting instrument. Using the proposed model, we applied a series of experiments. The results showed that the square axis has the best inhibitory effect on the proliferation of AC-16 cells; with an increase in stimulation time, the inhibitory effect improves. In addition, high-frequency mechanical stimulation was more effective than the low frequency in inhibiting the proliferation of AC-16 cell lines. This kind of myocardial cell physiological microenvironment model can record real-time myocardial cell mechanics model under the mechanical feedback signal, it can repeat the myocardial cell in vitro mechanical signal, and can effectively represent the mechanical properties of myocardial cells. It provides a new research method to further explore myocardial cell responses to stimulation.