MOESM5 of Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

Additional file 5. Degenerated oocysts of two chimeric P. falciparum parasite lines (pf-pvcsp) and a PfCSP knockout line (PfΔcsp). Light microscope pictures of degenerated oocysts at day 10 after feeding gametocytes to Anopheles stephensi mosquitoes. These oocysts are classified as degenerate based on the absence of sporozoite formation and vacuolated cytoplasm. See Fig. 2 for pf-pvcsp and wild type P. falciparum (WT) oocyst in which sporozoite formation occurred. No sporozoite formation was observed in PfΔcsp oocysts. Scale bar, 20 µm.