GC–MS analysis of bioactive constituents of Peristrophe bicalyculata (Retz.) Nees. (Acanthaceae)

Objective To characterize the chemical constituents of Peristrophe bicalyculata (P. bicalyculata) Received 9 January 2012 (Retz.) Nees. using GC-MS. Methods Ten grams of powdered sample was extracted with Received in revised form 7 February 2012 30 mL ethanol overnight and filtered through ash less filter paper with sodium sulphate (2 g) and the extract was concentrated to 1 mL by bubbling nitrogen into the solution. The Clarus 500 GC used in the analysis employed a column packed with Elite–1 (100% dimethyl poly siloxane, 30 nm × 0.25 nm ID. × 1 μm df) and the components were separated using Helium (1 mL/min) as the carrier gas. The 2 μL sample extract injected into the instrument was detected by the Turbo gold mass detector (Perkin Elmer) with the aid of the Turbo mass 5.1 software. Results The GC-MS analysis provided different peaks determining the presence of seven different phytochemical Phytochemistrycompounds namely propane,1,1-diethoxy (68.89%), (6Z)-nonen-1-ol (24.00%), 4-methyl-2,4Chemical profilebis(4'-trimethylsilyloxyphenyl)pentene-1 (3.56%), cyclooctyl alcohol (1.78%), oxirane, butyl-(0.89%), Medicinal plants (2H)pyrrole-2-carbonitrile,5-amino-3,4-dihydro-(0.44%) and ethaneperoxoic acid,1-cyano 1-[2-(2-phenyl-1,3-dioxolan-2-yl)ethyl] pentyl ester (0.44%). Conclusions The presence of various bioactive compounds confirms the application of P. bicalyculata for various ailments by traditional practitioners. However, isolation of individual phytochemical constituents may proceed to find a novel drug.