CHARACTERIZATION AND REGULATION OF IGFBP-4 IN CULTURED MOUSE LEYDIG CELLS (TM-3)

Authors :: Yukihiro Hasegawa
Ron G. Rosenfeld
Pinchas Cohen
Tomonobu Hasegawa
Paul J. Fielder
Source :: Pediatric Research. 33:S43-S43
Publication Year :: 1993
Publisher :: Springer Science and Business Media LLC, 1993.
Abstract: The characterization and regulation of IGFBPs in TM-3 cells was performed. Western Ligand blots of conditioned media(CM) demonstrated the presence of 24 and 28 kDa bands as major IGFBPs, and 40 and 44 kDa as minor ones. The 24 and 28 kDa bands were immunoprecipitated with an antibody to rat IGFBP-4. The 28 kDa band was deglycosylated with endoglycosidase-F to 24 kDa.

Subjects :: Blot
Immunoprecipitation
Pediatrics, Perinatology and Child Health
Conditioned medium
biology.protein
Biology
Antibody
Ligand (biochemistry)
Molecular biology

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