Studies on the Formation of Glucosamine Acid byAcetobacter melanogenumBeijerinck andPseudomonas fluorescens liquefaciens

d-Glucosaminic acid has recently been found to be an oxidized product of d-glucosamine formed by Ps. fluorescens. It has been revealed that many strains of oxidative bacteria can oxidize glucosamine. The formation of glucosamine acid has been recognized among a large number of strains of Pseudomonas, Acetobacter and Gluconobacter, by means of paper chromatography. Furthermore, one of these strains, A. melanogenum Beijerinck, oxidized glucosamine to glucosaminic acid with the theoretical consumption of oxygen as Ps. fluorescens liquefaciens. Glucosaminic acid was proved by isolation and identification by means of using resting cells.The experiment of growth shows that Ps. fluorescens liq. could not secure any energy by means of the oxidation of glucosamine.