Back to Search
Start Over
Transcriptional expression of Na+ homeostasis-related genes and physiological responses of rice seedlings under salt stress
- Source :
- Journal of Plant Biochemistry and Biotechnology. 30:81-91
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Being a salt-sensitive crop, the growth, development and productivity of rice is severely affected by saline soil. In the present study, Na+ homeostasis, physiological and morphological responses in both the root and leaf tissues of rice (Oryza sativa) seedlings of backcross introgression lines of KDML105 (KD; −/−) × FL530 (+/+) with or without SKC1 and qSt1b QTLs grown under 200 mM NaCl were investigated. Expression levels of OsHKT1;5 in FL530 (+/+) were very low, causing to low Na+ in both the root and leaf tissues, whereas it was up-regulated in KD (−/−), relating to Na+ enrichment. Expression levels of OsHKT2;1 in rice lines, 221-44 (+/+) and 221-48 (+/+) were down-regulated, thus limiting Na+ xylem upload, and store Na+ in the root tissues. OsSOS1 expression level in the leaves of 221-44 (+/+) and 221-58 (+/−) was up-regulated in relation to Na+ efflux. Na+ secretion into vacuoles via OsNHX1 in the leaf tissues was evidently demonstrated in 221-48 (+/+) as a major defense mechanism adopted by the plants to maintain photosynthetic abilities and growth performances, when plants were subjected to salt stress. In addition, the high Fv/Fm ratio in the leaves of 221-58 (+/−) was associated with increased expression level of OsNHX3, in comparison to KD (−/−) seedlings grown under salt stress (diminution by 22.8% over control). Moreover, reduction on growth characters and physiological parameters, viz., shoot fresh weight, shoot height, number of leaves, leaf area, Pn, and accumulation of Na+ in the roots under salt stress were applied as effective indices; thereby classifying FL530 (+/+) and 221-48 (+/+) as salt-tolerant, and 221-44 (+/+), 221-58 (+/−), KD (−/−), 221-3 (+/−), and 221-54 (−/−) as salt-susceptible.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Diminution
Soil salinity
Oryza sativa
fungi
food and beverages
Xylem
Plant Science
Vacuole
Biology
Photosynthesis
01 natural sciences
03 medical and health sciences
Horticulture
030104 developmental biology
Shoot
Agronomy and Crop Science
Homeostasis
010606 plant biology & botany
Biotechnology
Subjects
Details
- ISSN :
- 09741275 and 09717811
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Journal of Plant Biochemistry and Biotechnology
- Accession number :
- edsair.doi...........b5d21900c9c745ec3e45d354ef8ed955