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Polarimetric second harmonic generation microscopy: An analytical tool for starch bioengineering
- Source :
- Starch - Stärke. 70:1700031
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Second harmonic generation (SHG) is a nonlinear optical process that inherently generates signal in non-centrosymmetric materials, such as starch granules, and therefore can be used for label-free imaging. Both intensity and polarization of SHG are determined by material properties that are characterized by the nonlinear susceptibility tensor, χ(2). Examination of the tensor is performed for each focal volume (voxel) of the image by measuring the outgoing polarization state of the SHG signal for a set of incoming laser beam polarizations. Mapping of nonlinear properties expressed as the susceptibility ratio reveals structural features including the organization of crystalline material within a single starch granule, and the distribution of structural properties in a population of granules. Isolated granules, as well as in situ starch, can be analyzed using polarimetric SHG microscopy. Due to the fast sample preparation and short imaging times, polarimetric SHG microscopy allows for a quick assessment of starch structure and permits rapid feedback for bioengineering applications. This article presents the basics of SHG theory and microscopy applications for starch-containing materials. Quantification of ultrastructural features within individual starch granules is described. New results obtained by polarization resolved SHG microscopy of starch granules are presented for various maize genotypes revealing heterogeneity within a single starch particle and between various granules.
- Subjects :
- 0301 basic medicine
education.field_of_study
animal structures
Materials science
business.industry
Starch
Organic Chemistry
Population
Second-harmonic imaging microscopy
food and beverages
Second-harmonic generation
Second Harmonic Generation Microscopy
Polarization (waves)
Maize starch
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Optics
chemistry
Microscopy
education
business
Food Science
Subjects
Details
- ISSN :
- 00389056
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- Starch - Stärke
- Accession number :
- edsair.doi...........b4db69a7c9d07cf2e29756dbd030c225