The chromatography of some hydrazine derivatives on paper

A study is described of the chromatography on paper of some alkylhydrazines and some monoand diacylhydrazines by both the circular and ascending methods. For the acylhydrazines the solvent system preferred was the upper layer from isoamyl alcohol, acetic acid, and water in the volume proportions of 10:1.5 :10 respectively. The upper layer from a mixture of n -butyl alcohol, acetic acid, and water in the volume proportions of 4 : 1 : 5 respectively, was preferred for the alkylhydrazines. For detection of the hydrazine derivatives on the chromatogram a spray of p -dimethylaminobenzaldehyde hydrochloride in ethanol (Ehrlich's reagent) was most satisfactory, although ammoniacal silver nitrate or ninhydrin could be used in some cases.