Back to Search
Start Over
Investigation of techno-functional and physicochemical properties of Spirulina platensis protein concentrate for food enrichment
- Source :
- LWT. 114:108267
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The nutritional composition of Spirulina platensis has been studied extensively, especially its proteins, amino acids, vitamins and mineral content. However, research on its protein properties, including its techno-functional properties, remains scarce. Therefore, S. platensis proteins were extracted and concentrated to produce a S. platensis protein concentrate (SpPC), which was characterized by its physicochemical and techno-functional properties. Response surface methodology (RSM) was applied to evaluate the influence of two variables, pH and SpPC concentration, on the techno-functional properties of the SpPC, instead of studying one variable at a time. The SpPC contained 75.97% proteins (dry weight); its minimum solubility was at pH 3.0, and its maximum solubility was at pH 9.0. The effects of the SpPC concentration and pH on the water absorption capacity and emulsifying and foaming properties were evaluated. The pH exhibited a positive effect on these properties, and the best conditions were far from the isoelectric point. The SpPC concentration also showed a positive effect, except water and oil absorption. Considerable concentrations of amino acids were found in the S. platensis protein concentrate; tryptophan had the highest chemical score. The most abundant secondary structure conformation was β-sheet. Consequently, the S. platensis protein concentrate may contribute technologically to food formulation, and facilitate industrial processing.
- Subjects :
- 0106 biological sciences
chemistry.chemical_classification
Absorption of water
Chemistry
Tryptophan
04 agricultural and veterinary sciences
040401 food science
01 natural sciences
Amino acid
0404 agricultural biotechnology
Isoelectric point
Dry weight
010608 biotechnology
Spirulina (dietary supplement)
Response surface methodology
Food science
Solubility
Food Science
Subjects
Details
- ISSN :
- 00236438
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- LWT
- Accession number :
- edsair.doi...........b4ba4aae30d6fea428de11a84c959577