The Polymerase Chain Reaction (PCR): Solution PCR on Paraffin–Embedded Human Tissues

The polymerase chain reaction (PCR) is a valuable tool in the laboratory. PCR enables an investigator to amplify specific DNA and subsequently view the results of the amplification. There are many potential uses for PCR: forensics; identification of genetically inherited diseases; screening of genetically modified organisms; and classification and diagnosis of problematic, small tissue samples.Performing PCR on paraffin-embedded human tissues presents challenges. Fixation, microtomy, DNA extraction, master mix preparation, amplification, and gel electrophoresis are all intricate parts of the PCR process. This report outlines reliable, reproducible methods for achieving accurate PCR results on fixed, routinely processed, paraffinembedded human tissues. (The J Histotechnol 28:219, 2005)Submitted August 24, 2005; accepted with revisions September 28, 2005