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Okadaic acid induces appearance of the mitotic epitope MPM-2 in SV40-infected CV-1 cells with a >G2-phase DNA content
- Source :
- Cytometry. 31:260-264
- Publication Year :
- 1998
- Publisher :
- Wiley, 1998.
-
Abstract
- Simian virus 40 (SV40) infection of quiescent monkey kidney cells stimulates two successive rounds of cellular DNA synthesis without an intervening mitosis. This uncoupling of S phase and mitosis indicates that SV40 modulates pathways regulating the G2-to-M phase transition. To examine the integrity of mitotic initiation pathways in infected cells that have bypassed mitosis, SV40-infected CV-1 cells were treated with okadaic acid (OA), a known inducer of premature mitosis in other cell types. OA treatment triggered the appearance of the mitotic marker MPM-2 in SV40-infected CV-1 cells progressing through either the first (diploid) or second (tetraploid) S phases. These results demonstrate that a subset of mitotic pathways are intact but inactive in SV40-infected cells that have bypassed mitosis and initiated tetraploid S phase.
- Subjects :
- Cell type
medicine.diagnostic_test
viruses
Binucleated cells
Biophysics
Cell Biology
Hematology
Okadaic acid
Cell cycle
Biology
Molecular biology
Epitope
Pathology and Forensic Medicine
Cell biology
Flow cytometry
chemistry.chemical_compound
Endocrinology
chemistry
medicine
Endoreduplication
Mitosis
Subjects
Details
- ISSN :
- 10970320 and 01964763
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Cytometry
- Accession number :
- edsair.doi...........b38f3dd7051ad531b11fc58cc901f7bb