Affinity chromatography of carboxylic proteinases on a polymeric sorbent containing gramicidin-C as ligand

A new polymeric sorbent for proteinases has been synthesized by the radical copolymerization of N-vinylpyrrolidone, bis-Nδ-acryloylgramicidin C, and N,N′-methylenebisacrylamide. Biospecific chromatography on the new sorbent has enabled an industrial preparation of procine pepsin to be purified by a factor of 2.5. With the aid of the new sorbent, a carboxylic proteinase has been isolated from the industrial preparation Tsellolignorin with a 15-fold purification factor.