ENANTIOMERIC SEPARATION OF A DRUG SUBSTANCE USING CAPILLARY ELECTROPHORESIS WITH SULFATED-β-CYCLODEXTRIN

The enantioseparation of a weakly basic pharmaceutical drug substance is investigated using sulfated-β-CD as a chiral selector. The enantiomers are separated at acidic pHs in an anodic flow due to complexation with sulfated-β-CD, while under an electroosmotic flow (EOF) counter-current. At basic pHs, the EOF prevails and the analytes migrate toward the cathode, where improved separations can be obtained along with an apparent reversal of migration order. The optimal enantioseparation of the test compound is systematically explored by varying important factors, such as pH, concentration of sulfated-β-CD, temperature, and addition of organic modifier. The implications of the results on general enantioseparation of weakly basic pharmaceutical compounds are discussed.