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Exosome-modified PLGA Microspheres for Improved Internalization into Dendritic Cells and Macrophages
- Source :
- Biotechnology and Bioprocess Engineering. 25:521-527
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Considering the significance of effective antigen presentation for boosting immune responses, it is essential to develop delivery systems for antigen presenting cells (APCs; dendritic cells and macrophages). As a simple and facile way for improving delivery efficiency of PLGA microspheres (MS) into APCs, we fabricated exosome-conjugated PLGA MS via polydopamine coating in this study. Spherical micro-sized particles were first prepared by conventional water-in oil-in water (W1/O/W2) double emulsion and solvent evaporation methods and were observed by scanning electron microscopy (SEM). With increasing model protein (ovalbumin)/MS weight ratios, higher amounts of ovalbumin (OVA) were immobilized onto MS. After exosome (EXO) conjugation to MS via polydopamine coating, the amount of nitrogen was significantly increased on the surface of MS, indicating that EXO were successfully conjugated onto MS. EXO-coated dopamine MS (EXO-Dopa MS) exhibited significantly improved delivery into DC2.4 cells and RAW264.7 cells, compared with bare MS and Dopa MS. Therefore, EXO-Dopa MS could be used as effective carriers of immune stimulating biomolecules into APCs for cancer immunotherapy.
- Subjects :
- 0106 biological sciences
medicine.medical_treatment
media_common.quotation_subject
Antigen presentation
Biomedical Engineering
Bioengineering
01 natural sciences
Applied Microbiology and Biotechnology
Exosome
03 medical and health sciences
chemistry.chemical_compound
Immune system
Cancer immunotherapy
010608 biotechnology
medicine
Antigen-presenting cell
Internalization
030304 developmental biology
media_common
0303 health sciences
biology
Chemistry
Ovalbumin
PLGA
biology.protein
Biophysics
Biotechnology
Subjects
Details
- ISSN :
- 19763816 and 12268372
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Biotechnology and Bioprocess Engineering
- Accession number :
- edsair.doi...........b1ea1da5ff506836da3d7bafa14ddfa1