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FPX-113 attenuates inflammatory responses by deteriorating cytokines, neutrophil activity and mast cell degranulation via Akt/ NF- κB pathway
- Source :
- Biologia. 75:447-456
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Search of novel chemical compounds to manage disorders of inflammation is always on demand. Herein we report FPX-113 a novel small molecule to exhibit excellent anti-inflammatory properties in human whole blood (HWB), peripheral blood mononuclear cells (PBMCs), RBL-2H3 and THP-1 cells. FPX-113 showed an inhibition of TNF-α, IFN-γ, IL-2, IL-6, IL-8 and IL-17 release from HWB and PBMC with IC50 values of 364.6 nM, 505.2 nM, 160.2 nM, 369.1 nM 371.2 nM, 459 nM and 64.14 nM, 92.30 nM, 49.88 nM, 151.7 nM, 108.9 nM, 92.70 nM respectively. The compound inhibited neutrophil migration and elastase in a dose dependent way. Further, FPX-113 inhibited the degranulation of rat mast cell basophils in RBL-2H3 cells with an IC50 of 280.5 nM. A dose dependent inhibition of Akt phosphorylation in RBL-2H3 and THP-1 cells and down regulation of NF- κB in PBMCs, RBL-2H3 and THP-1 cells by FPX-113 were evidenced when induced with 5 μg/mL PHA + 50 ng/mL PMA for 30 min. Taken together, the results suggest FPX-113 exhibits anti-inflammatory mechanism signaled via Akt/ NF- κB pathway. These findings will provide a wide scope for FPX-113 and its close analogues to be developed as novel anti-inflammatory agents for various diseases.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Inflammation
Plant Science
Pharmacology
01 natural sciences
Biochemistry
Peripheral blood mononuclear cell
03 medical and health sciences
chemistry.chemical_compound
Downregulation and upregulation
Genetics
medicine
Molecular Biology
Protein kinase B
Ecology, Evolution, Behavior and Systematics
Chemistry
Elastase
Degranulation
hemic and immune systems
NF-κB
Cell Biology
Mast cell
030104 developmental biology
medicine.anatomical_structure
Animal Science and Zoology
medicine.symptom
010606 plant biology & botany
Subjects
Details
- ISSN :
- 13369563 and 00063088
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- Biologia
- Accession number :
- edsair.doi...........b1c8a05f50cc61f8848ef3cd67dcd55a