Back to Search
Start Over
Structural analysis of an innate immunostimulant from broccoli, Brassica oleracea var. italica
- Source :
- Drug Discoveries & Therapeutics. 11:230-237
- Publication Year :
- 2017
- Publisher :
- International Research and Cooperation Association for Bio & Socio-Sciences Advancement (IRCA-BSSA), 2017.
-
Abstract
- Vegetables are eaten as part of a healthy diet throughout the world, and some are also applied topically as a traditional medicine. We evaluated the innate immunostimulating activities of hot water extracts of various vegetables using the silkworm muscle contraction assay system, and found that broccoli, Brassica oleracea var. italica, contains a strong innate immunostimulant. We purified the innate immunostimulant from broccoli, and characterized the chemical structure by chemical analyses and NMR spectroscopy. The innate immunostimulant comprised galacturonic acid, galactose, glucose, arabinose, and rhamnose, and had a pectic-like polysaccharide structure. To determine the structural motif involved in the innate immunostimulating activity, we modified the structure by chemical and enzymatic treatment, and found that the activity was attenuated by pectinase digestion. These findings suggest that a pectic-like polysaccharide purified from broccoli has innate immune-stimulating activity, for which the polygalacturonic acid structure is necessary.
- Subjects :
- 0301 basic medicine
chemistry.chemical_classification
Arabinose
030102 biochemistry & molecular biology
biology
Rhamnose
medicine.drug_class
Chemical structure
food and beverages
General Medicine
biology.organism_classification
Polysaccharide
Immunostimulant
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
chemistry
Galactose
medicine
Brassica oleracea
Pharmacology (medical)
Food science
General Pharmacology, Toxicology and Pharmaceutics
Pectinase
Subjects
Details
- ISSN :
- 1881784X and 18817831
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Drug Discoveries & Therapeutics
- Accession number :
- edsair.doi...........b146b0ac6f0d1f5114d5a5842548f2cb