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Assessing a diagnosis tool for bacterial vaginosis
- Source :
- European Journal of Clinical Microbiology & Infectious Diseases. 39:1481-1485
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Diagnosis of bacterial vaginosis (BV) in resource-poor settings relies on semiquantitative microscopy algorithm such as the Nugent score (NS). We evaluated a quantitative real-time PCR (qPCR) assay to detect and quantify individual BV-associated bacterial communities. Vaginal swabs from 247 South African women attending an STI clinic were evaluated for BV using NS. We used qPCR to analyze DNA from vaginal swabs for eight BV-associated bacteria, Gardnerella vaginalis (GV), Prevotella bivia (PB), BV-associated bacteria 2 (BVAB2), Megasphaera-1 (M-1), Atopobium vaginae (AV), Lactobacillus crispatus (LC), Lactobacillus jensenii (LJ), and Lactobacillus iners (LI). Sensitivities and specificities were generated for each qPCR assay. Using a ROC analysis, cutoffs were calculated for each bacterial species. A logistic regression model was used to determine the strongest predictors of BV status. Nugent scores indicated 35.6% of patients harbor BV-associated flora (NS 7-10). AV, GV, GAMB (GV + AV + M-1 + BVAB2), and LC + LJ showed the highest AUC, sensitivities, and specificities (listed respectively): AV (0.96; 96%; 93%), GV (0.88; 78%; 79%), GAMB (0.9; 87%; 82%), and LC + LJ (0.84; 82%; 72%) (all p
- Subjects :
- 0301 basic medicine
Microbiology (medical)
medicine.medical_specialty
030106 microbiology
ved/biology.organism_classification_rank.species
Atopobium vaginae
medicine.disease_cause
Gastroenterology
Prevotella bivia
03 medical and health sciences
0302 clinical medicine
Internal medicine
medicine
Lactobacillus iners
Gardnerella vaginalis
030212 general & internal medicine
biology
Lactobacillus crispatus
Lactobacillus jensenii
ved/biology
business.industry
General Medicine
biology.organism_classification
medicine.disease
Infectious Diseases
Nugent score
Bacterial vaginosis
business
Subjects
Details
- ISSN :
- 14354373 and 09349723
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- European Journal of Clinical Microbiology & Infectious Diseases
- Accession number :
- edsair.doi...........b0d268f26066cc46b1dc2a6864f3dd28