[Untitled]

Authors :: Moshe White
I-Chun Kuo
Lin-Tang Goh
Kaw Yan Chua
Sa Luo
Source :: Biotechnology Letters. 23:661-665
Publication Year :: 2001
Publisher :: Springer Science and Business Media LLC, 2001.
Abstract: The gene of Blomia tropicalis group 5 allergen (Blo t 5) was cloned and expressed in Pichia pastoris KM71. Selected KM71 clones were cultivated in a fed-batch bioreactor feeding first glycerol then followed by methanol. Recombinant Blo t 5 constituted about 30% of the total broth protein after 60 h cultivation. The harvested broth was purified to >95% purity by a two-step anion exchange chromatography. The overall yield was 37 mg Blo t 5 per litre broth.