Evaluation of immunostimulatory properties of prickly chaff flower Achyranthes aspera in rohu Labeo rohita fry in pond conditions

The immunostimulatory properties of seeds and leaves of Achyranthes aspera (Linn.) was evaluated in rohu Labeo rohita in the pond conditions. Rohu fry (1.9 ± 0.08 g) were introduced in nine hapas (25 hapa−1) set inside a pond. Two test diets enriched with 0.5% seeds (D1) and leaves (D2) of A. aspera and control diet (D3) were fed for 60 days. Then fish were immunized with chicken-RBC and blood and tissue samples were collected on days-7, 14 and 21 after immunization. The average weight was significantly (p