Electron-cytochemical investigation of the brain mitochondria at various times after death

An electron-cytochemical investigation was made of oxidation of 3,3′-diaminobenzidine (DAB) in the brain of rats and man at different times after death. The oxidation product of DAB was localized in the mitochondria, lipofuscin granules, and erythrocytes. Oxidation of DAB by rat and human brain mitochondria was shown to be only very slightly depressed even 2 days after death.