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Paper microfluidic device enables rapid and on-site wastewater surveillance in community settings
- Publication Year :
- 2022
- Publisher :
- Research Square Platform LLC, 2022.
-
Abstract
- Tracking genomic sequences as microbial biomarkers in wastewater has been used to determine community prevalence of infectious diseases, contributing to public health surveillance programs. Here we report upon a low-cost, rapid, and user-friendly paper microfluidic platform for SARS-CoV-2 and influenza detection, using a loop-mediated isothermal amplification (LAMP), with the signal read simply using a mobile phone camera. Sample-to-answer results were collected in -1. The device was subsequently used for on-site testing of SARS-CoV-2 in wastewater samples from four quarantine hotels at London Heathrow Airport, showing comparable results to those obtained using a gold-standard polymerase chain reaction assay, as reference. Our sensing platform, which enables rapid and localized wastewater surveillance and does not require the sample to be sent to a centralized laboratory, is potentially an important public health tool for a wide variety of future applications, in community settings.
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi...........ae0f419ea46c8219bbd92ad7cfe94186